| Allergen: | Biotinylated nAra h 2 (Arachis hypogaea allergen 2) |
| Unit: | 1mg |
| Source: | Light roasted peanut flour (Runner cultivar) |
| Mol. Wt: | 17-19 kD doublet |
| Purification: | From delipidated peanut extract by multi-step chromatography. Purity on silver stained SDS-PAGE >95%. |
| Concentration: | See product insert. By Advanced Protein Assay (Cytoskeleton Inc., Denver, CO) |
| Formulation: | Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Sterile filtered. |
| Storage: | Store at -20ºC |
| Notes: | (1) Ara h 2 appears as a doublet (4). (2) Protect from exposure to light. (3) Avoid repeated freeze-thaw cycles. |
| PDB: | |
| Product Resources: | BiotinNatural Ara h 2 Certificate of Analysis |
| Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
| References: | |
Burks AW, Williams LW, Connaughton C, Cockrell G, O’Brien TJ, Helm RM. Identification and characterization of a second major peanut allergen, Ara h II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J Allergy Clin Immunol 1992;90:962-9. | |
Sen M, Kopper R, Pons L, Abraham EC, Burks W, Bannon GA. Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-Binding epitopes. J Immunol 2002;169:882-7. | |
| Flinterman AE, van Hoofen E, den Hartog Jager CF, Koppelman S, Pasmans SG, Hoekstra MO, Bruijnzeel-Koomen CA, Knulst AC, Knol EF. Children with peanut allergy recognize predominantly Ara h2 and Ara h 6, which remains stable over time. Clin Exp Allergy 2007;37:1221-8.. | |
McDermott RA, Porterfield HS, El Mezayen R, Burks AW, Pons L, Schlichting DG, Solomon B, Redzic, JS, Harbeck RJ, Duncan MW, Hansen KC, Dreskin SC. Contribution of Ara h 2 to peanut-specific, immunoglobulin E-mediated, cell activation. Clin Exp Allergy 2007;37:752-763. | |
