ELISA 2.0 kit for measuring Apium gravolensallergen, Api g 1 | |||||||
| Antibodies: | 5D4/7C4 | ||||||
| Standard Range: | 200-0.39 ng/mL | ||||||
| Limit of Detection: | 01.56 ng/mL | ||||||
| Background: | OD<0.08 at 450nm | ||||||
| Coefficient of Determination: | R-squared>0.98 | ||||||
| Contents: | Plates: Pre-coated with anti-Api g 1 monoclonal antibody 5D4Vial 1: (white top) Api g 1 allergen standardConcentration: 2,000 ng/mlVial 2: (brown) anti-Api g 1 antibody 7C4Vial 3: (blue top) Streptavidin-PeroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||||||
| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | 6 months from date of receipt | ||||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||||||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||||||
| Product Resources: | EPC-AG1-1 Certificate of Analysis Validation Performance Data | ||||||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||||||
| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 5D4 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||||||
| Monoclonal Antibody: | 5D4 | ||||||
| Immunogen: | Api g 1 | ||||||
| Isotype: | Mouse IgG1/kappa | ||||||
| Specificity: | Binds to species specific epitope present on Apium gravolensallergen, Api g 1. | ||||||
| Purification: | Produced in vitro cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Certificate of Analysis | |||||||
| Detection Antibody: | 7C4 | ||||||
| Immunogen: | Api g 1 | ||||||
| Isotype | Mouse IgG1/kappa | ||||||
| Specificity: | Binds to species specific epitope present on Apium gravolensallergen, Api g 1. | ||||||
| Purification: | Produced in vitro cell culture and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
| Certificate of Analysis | |||||||
| Allergen Standard: | Purified recombinant Api g 1 allergen 1.0101 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. | ||||||
| Concentration/Calibration: | 2,000 ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
