ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 8. | |||||||
| Antibodies: | 4G6/pAb | ||||||
| Standard Range: | 100-0.2ng/mL | ||||||
| Limit of Detection: | 0.78ng/mL | ||||||
| Background: | OD<0.08ng/ml at 450nm | ||||||
| Coefficient of Determination: | R-squared>0.98 | ||||||
| Contents: | Plate: Pre-coated with anti-Ara h 8 monoclonal antibody 4G6Vial 1: (white top) Ara h 8 allergen standardConcentration: 1,000 ng/mlVial 2: (brown) Rabbit polyclonal antiserumVial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit IgGBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||||||
| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | 6 months from date of receipt | ||||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||||||
| Notes: | The allergen standard is recommended for immunoassay calibration purposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||||||
| Product Resources: | EPC-AH8-1 Certificate of Analysis Validation Performance Data | ||||||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||||||
| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 4G6 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||||||
| Monoclonal Antibody: | 4G6 | ||||||
| Immunogen: | Ara h 8 | ||||||
| Isotype: | Mouse IgG1 | ||||||
| Specificity: | Binds to an epitope present on peanut allergen, Arachis hypogaea, Ara h 8. Minimal cross-reactive with structurally-homologous birch pollen allergen, Bet v 1. | ||||||
| Purification: | Produced in cell culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Certificate of Analysis | |||||||
| Detection Antibody: | Rabbit polyclonal antiserum | ||||||
| Immunogen: | Ara h 8 | ||||||
| Isotype | Multiple | ||||||
| Specificity: | The antiserum contains IgG antibodies to Ara h 8. Minimal cross-reactivity with structurally-homologous birch pollen allergen, Bet v 1. | ||||||
| Activity: | The antiserum has been titrated for use in ELISA at 1/1,000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | ||||||
| Certificate of Analysis | |||||||
| Allergen Standard: | Recombinant Ara h 8 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||||||
| Concentration/Calibration: | 1,000ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
