| ELISA 2.0 kit for measuring Felis domesticus allergen, Fel d 1. | ||||
| Antibodies: | 6F9/3E4 | |||
| Standard Range: | 25-0.05ng/mL | |||
| Limit of Detection: | 0.1ng/mL | |||
| Background: | OD<0.08 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plate: Pre-coated with anti-Fel d 1 monoclonal antibody 6F9Vial 1: (white top) Fel d 1 allergen standardConcentration: 250ng/mlVial 2: (brown) Biotinylated monoclonal antibody 3E4Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | |||
| Storage: | Store at 2-8°C | |||
| Expiry: | 6 months from date of receipt | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | |||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | |||
| Product Resources: | EPC-FD1-1 Certificate of Analysis Focus on... Fel d 1 Validation Performance Data | |||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 6F9 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | |||
| Monoclonal Antibody: | 6F9 (clone 6F9 A4 H1) | |||
| Immunogen: | Fel d 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1. | |||
| Purification: | Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE. | |||
| Certificate of Analysis | ||||
| Detection Antibody: | 3E4 (clone 3E4 C4 C10) | |||
| Immunogen: | Fel d 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to an epitope on cat Felis domesticus allergen, Fel d 1, Chain 1. | |||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Allergen Standard: | Purified natural Fel d 1 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | |||
| Concentration: | 250ng/mL (based on amino acid analysis) | |||
References: 1. Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TA. Monoclonal antibodies to the major feline allergen Fel d 1. 11. Single step affinity purification of Fel d 1, N terminal sequence analysis, and development of a sensitive two-site immunoassayto assess Fel d 1 exposure. J Immunol 1988; 140(3):812-818. 2. de Blay F, Chapman MD, Platts-Mills TA. Airborne cat allergen (Fel d 1).Environmental control with the cat in situ. Am Rev Respir Dis 1991; 143:1334-39. 3. Ingram JM, Sporik R, Rose G, Honsinger R, Chapman MD, Platts-Mills TA.Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens:relation to sensitization and asthma among children living in Los Alamos,NewMexico. J Allergy Clin Immunol 1995; 96(4):449-456. 4. Patchett K, Lewis S, Crane J, Fitzharris P. Cat Allergen (Fel d 1) levels on school children’s clothing and in primary school classrooms in Wellington, New Zealand. J Allergy Clin Immunol 1997; 100(6 Pt ):755-759 5. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoorallergens and asthma: report of the Third International Workshop. J Allergy ClinImmunol 1997; 100(6 Pt 1):S2-24. 6. Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation,asthma, and a modified Th2 response in children exposed to cat allergen: apopulation-based cross-sectional study. Lancet 2001; 357(9258):752-756. 7. Arbes SJ, Jr.,Cohn RD, Yin M, Muilenberg ML, Friedman W, Zeldin DC. Dogallergen (Can f 1) and cat allergen (Fel d 1) in US homes: results from the NationalSurvey of Lead and Allergens in Housing. J Allergy Clin Immunol 2004; 114(1):111-117. 8. Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681 9. Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dustmite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. JAllergy Clin Immunol. 2007 Mar; 119(3):697-704. | ||||
