ELISA 2.0 kit for measuring Rat Urinary Protein (RUP) allergen,Rat n 1. | |||
| Antibodies: | RUP-6/RUP-1 | ||
| Standard Range: | 50-0.1 ng/mL | ||
| Limit of Detection: | 0.39ng/mL | ||
| Background: | OD<0.08 at 450nm | ||
| Coefficient of Determination: | R-squared>0.98 | ||
| Contents: | Plate: Pre-coated with anti-Rat n 1 monoclonal antibody RUP-6Vial 1: (white top) Rat n 1 allergen standardConcentration: 500 ng/mlVial 2: (brown) Biotinylated monoclonal antibody RUP-1Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||
| Storage: | Store at 2-8°C | ||
| Expiry; | 6 months from date of receipt | ||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||
| Product Resources: | EPC-RN1-1 Certificate of Analysis Validation Performance Data | ||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||
| Certificate of Analysis | |||
| Monoclonal Antibody: | RUP-6 | ||
| Immunogen: | Rat n 1 | ||
| Isotype: | Mouse IgG1 | ||
| Specificity: | Binds to an epitope on rat Rattus norvegicus urinary allergen, Rat n 1. | ||
| Purification: | Produced in cell culture and purified by affinity chromatography using Protein G. | ||
| Certificate of Analysis | |||
| Detection Antibody: | RUP-1 | ||
| Immunogen: | Rat n 1 | ||
| Isotype: | Mouse IgG1 | ||
| Specificity: | Binds to an epitope on rat Rattus norvegicus urinary allergen, Rat n 1. | ||
| Purification: | Produced in cell culture and purified by affinity chromatography using Protein G. | ||
| Biontinylation: | Biotinylated and titrated for use in ELISA at 1/1000dilution. | ||
| Certificate of Analysis | |||
| Allergen Standard: | Purified natural Rat n 1 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||
| Concentration: | 500 ng/mL (based on amino acid analysis) | ||
References: 1. Renstrom A, Larsson PH, Malmberg P, Bayard C. A new amplified monoclonal rat allergen assay used for evaluation of ventilation improvements in animal rooms. J Allergy Clin Immunol. 1997 Nov;100(5):649-55. 2. Renstrom A, Gordon S, Larsson PH, Tee RD, Newman Taylor AJ, Malmberg P. Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement. Clin Exp Allergy. 1997 Nov;27(11):1314-21. | |||
